Influence of pulling handles and device stiffness in single-molecule force spectroscopy
نویسندگان
چکیده
منابع مشابه
Influence of pulling handles and device stiffness in single-molecule force spectroscopy.
In single-molecule force spectroscopy, individual molecules and complexes are often stretched by pulling devices via intervening molecular handles. Accurate interpretation of measurements from such experiments in terms of the underlying energy landscape, defined by activation barriers and intrinsic rates of transition, relies on our understanding, and proper theoretical treatment, of the effect...
متن کاملPulling geometry-induced errors in single molecule force spectroscopy measurements.
In AFM-based single molecule force spectroscopy, it is tacitly assumed that the pulling direction coincides with the end-to-end vector of the molecule fragment being stretched. By systematically varying the position of the attachment point on the substrate relative to the AFM tip, we investigate empirically and theoretically the effect of the pulling geometry on force-extension characteristics ...
متن کاملModel accounting for the effects of pulling-device stiffness in the analyses of single-molecule force measurements.
Single-molecule force spectroscopy provides a powerful approach for investigating molecular transitions along specific reaction coordinates. Here, we present a general analytical model for extracting the intrinsic rates and activation free energies from force measurements on single molecules that is applicable to a broad range of pulling speeds and device stiffnesses. This model relaxes existin...
متن کاملMinimizing pulling geometry errors in atomic force microscope single molecule force spectroscopy.
In atomic force microscopy-based single molecule force spectroscopy (AFM-SMFS), it is assumed that the pulling angle is negligible and that the force applied to the molecule is equivalent to the force measured by the instrument. Recent studies, however, have indicated that the pulling geometry errors can drastically alter the measured force-extension relationship of molecules. Here we describe ...
متن کاملOn artifacts in single-molecule force spectroscopy.
In typical force spectroscopy experiments, a small biomolecule is attached to a soft polymer linker that is pulled with a relatively large bead or cantilever. At constant force, the total extension stochastically changes between two (or more) values, indicating that the biomolecule undergoes transitions between two (or several) conformational states. In this paper, we consider the influence of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Phys. Chem. Chem. Phys.
سال: 2011
ISSN: 1463-9076,1463-9084
DOI: 10.1039/c0cp01528h